FIGURE 4.

Cross reactivity to APLs of the peptide SYIPSAEKI induced by nanovaccine systems. BALB/c mice were immunized once (A), or twice (B) with KI peptide alone, or either mixed with, or conjugated to, PSNPs (25 μg peptide, 1% PSNPs /mouse for both mixed in and conjugated groups). Fourteen days after the last immunization splenocytes were harvested and antigen specific or cross reactive IFN-γ production was measured via ELISpot assay. Data shown as mean ± SD of SFU/million cells (pooled cells from three mice per group) from triplicate wells. **p < 0.01, ***p < 0.001, ****p < 0.0001