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. 2016 Mar 11;7:311. doi: 10.3389/fmicb.2016.00311

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Copyright © 2016 Borba-Santos, Visbal, Gagini, Rodrigues, de Camargo, Lopes-Bezerra, Ishida, de Souza and Rozental.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mitochondrial activity after treatment of Sporothrix schenckii ATCC MYA 4821 and S. brasiliensis ATCC MYA 4823 yeast cells with H3 or itraconazole. Cells untreated or treated for 96 h with sub-inhibitory concentrations of H3 or itraconazole were stained with MitoTracker Red CMXRos, after which fluorescence intensity was analyzed by flow cytometry. H3 exposure induced a statistically significant fluorescence intensity increase (S. schenckii 4821) or decrease (S. brasiliensis 4823), reflecting mitochondrial disturbance (^∗p < 0.05).