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. 2016 Mar 11;7:287. doi: 10.3389/fpls.2016.00287

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Copyright © 2016 Qi, Yang, Wen, Hong, Song, Zhang and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Average expression stability values (M) and pairwise variation (V) of the candidate reference genes were calculated using GeNorm. (A) Average expression stability values (M) following stepwise exclusion of the least stable gene across all treatment groups. The least stable genes are on the left, while the most stable genes are on the right. (B). The optimal number of reference genes required for effective normalization. The pairwise variation (V_n/V_n₊₁) was analyzed between the normalization factors NF_n and NF_n₊₁ using GeNorm.