Table 4.

Reference gene primer sequences and amplicon characteristics.

Gene symbol	Gene description	Forward primer sequences (5′–3′)	Reverse primer sequences (5′–3′)	Amplicon Length (bp)	Tm (°C)	PCR efficiency (%)	Regression Coefficient (R2)	Mean of Cq value	References
Actin	Actin related protein 2	TAAGAACGATAAGTGCCCACATAG	TTTTAGACATCAGCCATAACAGAGT	201	62.1	122.7	0.992	27.76 ± 1.32	Fu et al., 2013
EF-1α	Elongation factor 1 alpha	TGCCGTTGGGGTGAGTATT	CCTTCTTTTCCTCCTCGGTCT	179	57	109.3	0.995	22.97 ± 1.35
PP2A	Protein phosphatase 2A	CGTGGGTCCTCAGAATCAAA	TGTCAGCCATCTGTAAAATG	297	55.1	93.6	0.994	25.24 ± 1.15	Hong and Dai, 2015
SAND	SAND family protein	CGTTGCTCACTACGAGTTCAC	GCAGATGGGTCAACAGGTAA	178	62.1	124.0	0.995	26.04 ± 1.08	Fu et al., 2013
MTP	Protein degradation	GGTGTTATGATTGGTGCTGCT	ATCTATCTCTCGTGGGGTGCT	184	57.2	131.6	0.995	25.11 ± 1.29	Fu et al., 2013
PGK	Phosphoglycerate kinase	ATACCTAATGGACGAAGAGA	TTATTGTCGTCTTTACTACCA	248	64.1	113.5	0.999	27.54 ± 1.19	Fu et al., 2013
TUB	beta tubulin	ATCAACTACCAGCCACCAAC	GAACTCACCTTCCTCCATACC	211	58	107.9	0.990	22.09 ± 1.28
UBQ	ubiquitin extension protein	GAACCATCCACGAACAATACAAGAGC	CAGGCTAAGAGGAGGGATGC	283	56.2	110.7	0.999	19.29 ± 1.20
F-box	F-box protein	CTGCTTTATGTATCGGAGGGA	AACGAAAACGAGAGGGGCT	246	58.3	95.9	0.995	23.15 ± 1.30