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. 2016 Jan 8;100:3219–3231. doi: 10.1007/s00253-015-7266-x

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Transformation efficiency of D. bruxellensis ura3 and ura5 mutants by linear and circular plasmids. Circular and by HindIII linearized plasmids were used for transformation. P892 plasmid is carrying D. bruxellensis URA3 gene, P1228—S. cerevisiae URA5 gene. a Transformation efficiency. b Colonies of transformants on selective medium YNB with 2 % glucose