Table 2.
The influence of acute administration of 1MeTIQ on changes induced by chronic treatment with 1BnTIQ in the concentration of dopamine (DA) and its metabolites
| Treatment | N | DA (ng/g) | DOPAC (ng/g) | 3-MT (ng/g) | HVA (ng/g) | |
|---|---|---|---|---|---|---|
| Acute | Chronic | |||||
| Striatum | ||||||
| Saline | Saline | 7 | 9894 ± 618 | 2158 ± 113 | 416 ± 30 | 1027 ± 90 |
| 1MeTIQ/25 | Saline | 7 | 11055 ± 264 | 1432 ± 61** | 620 ± 34** | 288 ± 27** |
| 1MeTIQ/50 | Saline | 7 | 10979 ± 502 | 1144 ± 47** | 706 ± 38** | 1049 ± 87 |
| Saline | MeTIQ/25 | 6 | 7416 ± 356** | 1999 ± 142 | 388 ± 16 | 1383 ± 146* |
| 1MeTIQ/25 | 1BnTIQ/25 | 7 | 9649 ± 542++ | 1254 ± 64**++ | 587 ± 45**++ | 1072 ± 58+ |
| 1MeTIQ/25 | 1BnTIQ/25 | 7 | 9105 ± 118++ | 968 ± 79**++ | 703 ± 44**++ | 881 ± 60++ |
| Effect of 1MeTIQ |
F
(2/35) = 8.88 P < 0.0007 |
F
(2/35) = 64.73 P < 0.0000001 |
F
(2/35) = 36.49 P < 0.0000001 |
F
(2/35) = 18.87 P < 0.000003 |
||
| Effect of 1BnTIQ |
F
(2/35) = 29.41 P < 0.00004 |
F
(2/35) = 5.11 P < 0.03 |
F
(2/35) = 0.50 NS |
F
(2/35) = 20.97 P < 0.00005 |
||
| Interaction of 1MeTIQ + 1BnTIQ |
F
(2/35) = 0.79 NS |
F
(2/35) = 0.01 NS |
F
(2/35) = 0.09 NS |
F
(2/35) = 14.88 P < 0.00002 |
||
| Nucleus accumbens | ||||||
| Saline | Saline | 7 | 9089 ± 406 | 2742 ± 98 | 320 ± 19 | 1049 ± 69 |
| 1MeTIQ/25 | Saline | 7 | 9796 ± 388 | 1742 ± 89** | 386 ± 18 | 901 ± 71 |
| 1MeTIQ/50 | Saline | 7 | 9765 ± 1145 | 1406 ± 204** | 402 ± 47 | 854 ± 129 |
| Saline | 1BnTIQ/25 | 6 | 6172 ± 358** | 2024 ± 94** | 233 ± 16* | 926 ± 53 |
| 1MeTIQ/25 | 1BnTIQ/25 | 7 | 8316 ± 413+ | 1378 ± 85**++ | 373 ± 24++ | 693 ± 44**+ |
| 1MeTIQ/50 | 1BnTIQ/25 | 7 | 8351 ± 476+ | 1080 ± 95**++ | 467 ± 34**++ | 664 ± 56**+ |
| Effect of 1MeTIQ |
F
(2/35) = 4.40 P < 0.01 |
F
(2/35) = 54.39 P < 0.0000001 |
F
(2/35) = 17.01 P < 0.000007 |
F
(2/35) = 5.80 P < 0.006 |
||
| Effect of 1BnTIQ |
F
(2/35) = 17.97 P < 0.0001 |
F
(2/35) = 25.95 P < 0.00001 |
F
(2/35) = 0.27 NS |
F
(2/35) = 8.75 P < 0.005 |
||
| Interaction of 1MeTIQ + 1BnTIQ |
F
(2/35) = 1.17 NS |
F
(2/35) = 1.86 NS |
F
(2/35) = 3.74 P < 0.03 |
F
(2/35) = 0.19 NS |
||
1BnTIQ was chronically administered (25 mg/kg i.p.) during 14 consecutive days. In the mixed group, 1MeTIQ (50 mg/kg i.p.) was given once, 20 min before last 1BnTIQ administration. Rats were decapitated 2 h after the injections. The results are expressed as the means ± SEM of six to seven samples (n = 6–7 animals per group). Data were analyzed with a two-way ANOVA followed by Duncan’s post hoc test. Statistical significance: * P < 0.05; ** P < 0.01 versus Saline/Saline group; + P < 0.05, ++ P < 0.01 versus 1BnTIQ group