Table 3.
The influence of chronic administration of 1MeTIQ on changes induced by chronic treatment with 1BnTIQ in the concentration of dopamine (DA) and its metabolites
| Treatment | N | DA (ng/g) | DOPAC (ng/g) | 3-MT (ng/g) | HVA (ng/g) | |
|---|---|---|---|---|---|---|
| Chronic | Chronic | |||||
| Striatum | ||||||
| Saline | Saline | 7 | 9894 ± 618 | 2158 ± 113 | 416 ± 30 | 1027 ± 90 |
| 1MeTIQ/25 | Saline | 7 | 11441 ± 254* | 1255 ± 59** | 603 ± 17** | 930 ± 47 |
| 1MeTIQ/50 | Saline | 7 | 11259 ± 579* | 976 ± 55** | 737 ± 35** | 868 ± 48 |
| Saline | 1BnTIQ/25 | 7 | 7416 ± 356** | 1999 ± 142 | 388 ± 16 | 1383 ± 146** |
| 1MeTIQ/25 | 1BnTIQ/25 | 7 | 8390 ± 239* | 1029 ± 49**++ | 616 ± 19**++ | 853 ± 59++ |
| 1MeTIQ/50 | 1BnTIQ/25 | 7 | 9924 ± 416++ | 823 ± 51**++ | 705 ± 37**++ | 807 ± 53++ |
| Effect of 1MeTIQ |
F
(2/36) = 10.18 P < 0.0003 |
F
(2/36) = 104.27 P < 0.0000001 |
F
(2/36) = 70.60 P < 0.0000001 |
F
(2/36) = 11.65 P < 0.0001 |
||
| Effect of 1BnTIQ |
F
(2/36) = 41.37 P < 0.0000001 |
F
(2/36) = 6.49 P < 0.01 |
F
(2/36) = 0.48 NS |
F
(2/36) = 1.18 NS |
||
| Interaction of 1MeTIQ + 1BnTIQ |
F
(2/36) = 2.01 NS |
F
(2/36) = 0.108 NS |
F
(2/36) = 0.41 NS |
F
(2/36) = 4.47 P < 0.01 |
||
| Nucleus accumbens | ||||||
| Saline | Saline | 7 | 9089 ± 406 | 2742 ± 98 | 320 ± 19 | 1049 ± 69 |
| 1MeTIQ/25 | Saline | 7 | 9552 ± 669 | 1295 ± 105** | 354 ± 25 | 689 ± 79** |
| 1MeTIQ/50 | Saline | 7 | 8892 ± 363 | 1050 ± 60** | 405 ± 29* | 593 ± 33** |
| Saline | 1BnTIQ/25 | 7 | 6172 ± 358** | 2024 ± 94** | 233 ± 16* | 926 ± 53 |
| 1MeTIQ/25 | 1BnTIQ/25 | 7 | 6971 ± 373**++ | 1038 ± 74**++ | 369 ± 22++ | 518 ± 43**++ |
| 1MeTIQ/50 | 1BnTIQ/25 | 7 | 8442 ± 581++ | 871 ± 77**++ | 393 ± 28*++ | 509 ± 24**++ |
| Effect of 1MeTIQ |
F
(2/36) = 2.43 NS |
F
(2/36) = 159.28 P < 0.0000001 |
F
(2/36) = 14.17 P < 0.00002 |
F
(2/36) = 39.26 P < 0.0000001 |
||
| Effect of 1BnTIQ |
F
(2/36) = 26.21 P < 0.00001 |
F
(2/36) = 29.92 P < 0.000004 |
F
(2/36) = 2.05 NS |
F
(2/36) = 8.25 P < 0.006 |
||
| Interaction of 1MeTIQ + 1BnTIQ |
F
(2/36) = 3.98 P < 0.02 |
F
(2/36) = 5.74 P < 0.006 |
F
(2/36) = 2.48 NS |
F
(2/36) = 0.33 NS |
||
1BnTIQ was chronically administered (25 mg/kg i.p.) during 14 consecutive days. In the mixed group, 1MeTIQ (50 mg/kg i.p.) was given also chronically, 20 min before each 1BnTIQ administration. Rats were decapitated 2 h after the injections. The results are expressed as the means ± SEM of seven samples (n = 7 animals per group). Data were analyzed with a two-way ANOVA followed by Duncan’s post hoc test. Statistical significance: * P < 0.05; ** P < 0.01 versus Saline/Saline group; + P < 0.05; ++ P < 0.01 versus 1BnTIQ group