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. 2015 Feb 2;13(2):170–179. doi: 10.1038/cmi.2015.02

Figure 1.

Figure 1

CORM-2, CoPP and hemin increase IRG1 expression in RAW264.7 macrophages. (a) RAW264.7 cells were treated with 20 µM CORM-2 for 0, 2, 4, 8, 16 and 24 h and protein level of IRG1 and HO-1 were detected by western blot analysis. (b and c) RAW264.7 cells were treated with CORM-2 (0, 5, 10, 20 and 40 µM) for 8 or 16 h. IRG1 and HO-1 mRNA and protein were measured, by RT-PCR analysis. (d–g) RAW264.7 cells were treated with CoPP or hemin (0, 1, 5, 10 and 20 µM) for 8 or 16 h. (d) After CoPP treatment at the indicated concentrations (0–20 µM) for 8 h, mRNA expression of IRG1 and HO-1 were detected. (e) After CoPP treatment (0–20 µM) for 16 h, IRG1 and HO-1 protein level were detected. (f) After hemin treatment at the indicated concentrations (0–20 µM) for 8 h, mRNA expression of IRG1 and HO-1 were detected. (g) After hemin treatment (0–20 µM) for 16 h, IRG1 and HO-1 protein level were detected. Protein level was detected by western blot analysis and mRNA levels were measured by RT-PCR analysis. Representative bands are shown.