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. 2015 Mar 9;13(2):229–239. doi: 10.1038/cmi.2015.08

Figure 1.

Figure 1

Analysis of activation and costimulatory molecule expression of viable B-1a cells. (a) The cells were cultured in either the absence or presence of IL-15 and tested at each time point as indicated for viability by the Trypan blue exclusion and MTT assay methods. (b) The cells were cultured with IL-15 (black line), anti-IgM Ab (gray line), compared with the untreated control (shaded) and analyzed by flow cytometry for the expression of CD69 (at 96 h), CD25 (at 24 h) and CD40 (at 24 h). (c) The cells were analyzed by flow cytometry for the expression of CD80 and CD86 (at 48 h) after culturing with either IL-15 (black line), anti-IgM Ab (gray line) or complete medium alone (shaded) (d) The flow cytometric analysis of IL-15 (thick black line) and LPS (thin black line)-induced expression of Siglec-G compared to the untreated control (gray line). The shaded profile indicates the isotype-matched control. The data shown are representative of three independent experiments. The bar diagrams represent the mean±s.e.m. of three separate experiments. *P<0.05, **P<0.005. LPS, lipopolysaccharide; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; Siglec, sialic acid-binding immunoglobulin-like lectin.