Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 7;7:10872. doi: 10.1038/ncomms10872

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Representative biophotonic images of the tissue distribution of fluorescence signal in mice at 4 and 8 h after intravenous injection of purified PKH67-labelled exosomes isolated from the supernatant of OC-miR-214-3p osteoclasts (OC-Exo). (b) Representative biophotonic images of the tissue distribution of fluorescence signal in mice at 8 h after intravenous injection of purified PKH67 exosomes isolated from the supernatant of either OC-miR-214-3p osteoclast (OC-Exo) or HEK 293T cells (HEK-Exo). (c) Western blot analysis of the Sema4D protein expression in pellets of sucrose gradient fractions from the osteoclast-derived exosome preparations. The density of each fraction was determined by refraction index measurements. (d,e) Flow cytometry analysis of PKH67⁺ osteoblasts after incubation with PKH67-labelled exosomes derived from OC-miR-214-3p osteoclasts. The PKH67-labelled exosomes were pre-treated with either anti-Sema4D (20 μg ml⁻¹) or isotype-matched control (Sema4D iso) and then added to osteoblasts for 4 h incubation. (f) Real-time PCR analysis of the levels of pri-miR-214, pre-miR-214 and mature miR-214 in osteoblasts after incubation with either anti-Sema4D-treated exosomes (OC Exo+Anti-Sema4D) or Sema4D isotype-treated exosomes (OC Exo+Sema4D iso), respectively. All data are the mean±s.d. of four independent experiments. *P<0.05. One-way ANOVA with a post-hoc test was performed.