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. 2016 Jan 12;291(11):5452–5460. doi: 10.1074/jbc.M115.672733

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Both OGG1 and UNG2 impede CREB1 from binding to a CRE site substrate when the CRE site contains the specific DNA lesion recognized by each glycosylase. Interference depended on the lesion, concentration of glycosylase, and order of exposure. A–C, OGG1 in increasing concentrations competes with CREB1 for an °G-containing substrate. A, OGG1 added before CREB1; B, CREB1 added before OGG1; C, OGG1 and CREB1 added together. Total bound CREB1 measured in the control experiments was 0.15 ± 0.01 nm (n = 9). D–F, CREB1 and UNG2 each prevent binding of the other. D, UNG2 added before CREB1; E, CREB1 added before UNG2; F, UNG2 and CREB1 added together. Total CREB1 bound in these control experiments was 0.35 ± 0.01 nm (n = 9).