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. 2016 Jan 12;291(11):5452–5460. doi: 10.1074/jbc.M115.672733

FIGURE 3.

FIGURE 3.

CREB1 dimerization is heavily impacted by the presence of DNA repair glycosylases. As the concentration of glycosylase was increased, CREB1 dimerization decreased, except for when the G/U mispair-containing CRE site had prior exposure to CREB1. A–C, regardless of whether the substrate was exposed to OGG1 before or after CREB1, total CREB1 dimerization was reduced up to 69% ± 2% (n = 3) relative to control. A, OGG1 added before CREB1; B, CREB1 added before OGG1; C, OGG1 and UNG2 added together. CREB1 dimerization was significantly reduced when OGG1 and CREB1 had concurrent access to the substrate as compared with when OGG1 had prior access to the substrate (p = 0.012, 3). CREB1 dimer in the control experiments was 0.02 nm ± 0.003 nm (n = 9). D–F, dimerization of CREB1 on a G/U mispair-containing CRE site. D, UNG2 added before CREB1; E, CREB1 added before UNG2; F, UNG2 and CREB1 added together. Measured CREB1 dimer in control experiments was 0.14 ± 0.01 nm (n = 9).