FIGURE 7.

Only the G/U mispair within the CpG islet of the CRE site causes developmental defects in surviving 24 hpf zebrafish embryos. Embryos were microinjected with the indicated 39-mer ds oligonucleotide substrate (Table 1). Viability and developmental progress were determined at 24 hpf. No differences from control were noted except for those microinjected with the CRE site containing a G/U mispair (31% ± 11%, n = 8). Control consisted of phenol red in 1× Danieau buffer. Error bars represent ± S.E., **, p ≤ 0.01.