FIGURE 8.

Developmental defects occur in zebrafish embryos microinjected with G/U mispair-containing CRE site oligonucleotide. A, control zebrafish embryos microinjected with 1× Danieau buffer and 0.05% phenol red dye displayed normal development; the lone exception (lower left in this image) displays curvature of the anterior-posterior axis. B, zebrafish embryos at 24 hpf displayed developmental defects of varying degrees after microinjection with a decoy CRE site oligonucleotide containing a G/U mispair in the CpG islet. Defects included kinked tail ends, curvature of anterior-posterior axis, and abnormal yolk extension. * denotes abnormal development.