TABLE 1.
Modifications made to CRE site used in this study
The Modification column shows the actual residue modification made to the CRE site. The residues and positions modified within the CRE site are denoted in the Location column. Modifications were made to, or opposite, the G2 residue within the CpG islet. The Strand column indicates the modified strand. T = top strand; B = bottom strand. The Sequence column shows the complete CRE site sequence including the modified residue(s). The full-length unmodified 39-mer CRE oligonucleotide is shown with the CRE site in bold in the bottom row. Numerals 1 and 2 indicate the guanine residues within the top strand of the CRE site. All modifications in this study were located at the G2 position or its complement. NA, not applicable.
| Modification | Location | Strand | Sequence |
|---|---|---|---|
| CRE | NA | T | T: 5′-TG ACG TCA- |
| B: 3′-AC TGC AGT- | |||
| 8-oxoguanine (oG) | G2 (CpG) | T | T: 5′-TG ACoG TCA- |
| B: 3′-AC TGC AGT- | |||
| G/U mispair (G/U) | G2 (CpG) | B | T: 5′-TG ACG TCA- |
| B: 3′-AC TGU AGT- | |||
| Scrambled CRE | Entire CRE-site | Both | T: 5′-GT CAT GAC- |
| B: 3′-CA GTA CTG- | |||
| Scrambled CRE (G/U) | Entire CRE-site U in bottom strand | Both | T: 5′-GT CAT GAC- |
| B: 3′-CA GTA UTG- | |||
| Full oligonucleotide sequence | 5′-TAC CAT GCC TTG1 ACG2 TCA GAG AGC ATT CGT AAT CAT GGT-3′ | ||