FIGURE 1.

Functionality of Slt2-as is inhibited by 2,3-DMB-PP1. A, 10-fold serial dilutions of the slt2Δ strain Y00993 transformed with the empty vector pRS316 (slt2Δ), pRS316-SLT2 (SLT2), or pRS316-slt2-as (slt2-as) were spotted onto YPD plates in the absence (No addition) or presence of Congo red (10 μg/ml) and incubated at 30 °C for 48 h. B, graphic showing the effect of distinct Congo red concentrations on growth in liquid medium after 48 h of the same transformants as in A. Results are the mean of three independent transformants. Error bars indicate S.D. C, graphic showing the effect of the indicated concentrations of 2,3-DMB-PP1 on growth of slt2Δ strain Y00993 transformed with plasmids pRS316-SLT2 (blue line) or pRS316-slt2-as (red line). Experiments were performed and data processed as in B.