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Determination of Slt2 phosphorylation sites on Caf20 and Rcn2. Western blot analysis of the in vitro kinase assay similar to Fig. 3B but with E. coli extracts containing recombinant GST-Caf20 and GST-Caf20T102A (A) or GST-Rcn2, GST-Rcn2S152A, GST-Rcn2S160A, GST-Rcn2S255A, and GST-Rcn23S-A (B) as substrates. Anti-thiophosphate ester antibodies (upper panels) and anti-GST (lower panels) were used to detect thiophosphorylated proteins and GST-tagged proteins respectively.
