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Genetic interaction of gga1Δ, gga2Δ, and slt2Δ mutations in the absence and presence of cell wall stress. A, 10-fold serial dilutions of the BY4741 cell and the isogenic mutants strains gga1Δ, gga2Δ, gga1Δ gga2Δ, slt2Δ, gga1Δ slt2Δ, and gga2Δ slt2Δ. The cells were spotted onto YPD plates in the absence (No addition) or presence of Congo red (1 and 10 μg/ml), zymolyase (10 and 50 μg/ml), caffeine (1 and 4 mm), and SDS (0.01%) and incubated at 30 °C for 48 h.
