FIGURE 9.

Carboxypeptidase (CPY) secretion and alkaline phosphatase (AP) release of gga1Δ, gga2Δ, and slt2Δ mutants. A, patches of the BY4741 wild-type strain (WT) and the isogenic mutants gga1Δ, gga2Δ, gga1Δ gga2Δ, slt2Δ, gga1Δ slt2Δ, and gga2Δ slt2Δ were grown on YPD medium (right panel) and blotted onto a nitrocellulose membrane followed by immunodetection of the membrane with anti-CPY antibodies (left panel). B, dot blotting analysis of CPY secretion (black and white photograph) performed as in A and AP release detection in the indicated wild-type and isogenic slt2Δ strains at 30 °C and 37 °C (color photographs). For AP analysis, the patches were replicated to YPD supplemented with BCIP and incubated at 30 °C or 37 °C for 24 h. C, AP release analysis, performed as in B, in the same strains as in A.