TABLE 1.

Oligonucleotides used in this work

Restriction sites are underlined, and mutated codons are in bold.

Name	Primers
FwSLT2	5′-CAATTCTGGGAGATGGCTG-3
RvSLT2	5′-CAAACTTCCGCGGAGTACG-3
Fwslt2E108G	5′-GGACTATATCTTTATGGTGAACTTATGGAATGTG-3′
Rvslt2E108G	5′-CACATTCCATAAGTTCACCATAAAGATATAGTCC-3′
FwNco1-BamH1 SLT2	5′-CCATGGGATCCATGGCTGATAAGATAGAGAG-3′
RvBamH1STOPNco1 SLT2	5′-GGATCCTAACCCATGGAAAAATATTTTCTATCTAATCCAAAC-3′
FwBamH1-msg51–227	5′-CCCGGGATCCATGCAATTTCACTCAG-3′
RvBamH1-msg51–227	5′-CCCCGGGTCAGGATCCCGTTCGTTGATAAACTCTTGC-3′
FwBamH1 msg5228–489	5′-CCCCCCGGGGATCCAGGAAGAAACATCATCTG-3′
RvBamH1-msg5228–489	5′-CCCCCGGGATCCGTTTATTTAGATGGACCATTG-3′
FwBamH1 GGA1	5′-CCGGATCC ATGCCACAAAGAATTGAGCTTAC-3′
RvXba1 GGA1	5′-CCTCTAGATTATATTGTGGGCAAACTGGTC-3′
FwBamH1 SOD1	5′-CCGGATCCATGGTTCAAGCAGTCGCAG-3′
RvEcoR1 SOD1	5′-CCGAATTCTTAGTTGGTTAGACCAATGAC-3′
FwBamH1 RCN2	5′-CCGGATCCATGGCAAACCAAAAGCAAATG-3′
RvEcoR1 RCN2	5′-CCGAATTCCTAATGGAAAAACTCGTTAAC-3′
Fwrcn2S255A	5′-GCCTCAAATCCTCCAAAAGCTCCAAGCATAACGGTTAAC-3′
Rvrcn2S255A	5′-GTTAACCGTTATGCTTGGAGCTTTTGGAGGATTTGAGGC-3′
Fwrcn2S152A/S160A	5′-CTCTTCATTAGCTCCGGATAAATCATCTCTAGAAGCGCCCACAATG-3′
Rvrcn2S152A/S160A	5′-CATTGTGGGCGCTTCTAGAGATGATTTATCCGGAGCTAATGAAGAG-3′
Fwrcn2S152A	5′-ATAAGGGAGGCTCTTCATTAGCTCCGGATAAATCATCTCTAGA-3′
Rvrcn2S152A	5′-TCTAGAGATGATTTATCCGGAGCTAATGAAGAGCCTCCCTTAT-3′
Fwrcn2S160A	5′-CGGATAAATCATCTCTAGAAGCGCCCACAATGTTGAAGCTTTC-3′
Fwrcn2S160A	5′-GAAAGCTTCAACATTGTGGGCGCTTCTAGAGATGATTTATCCG-3′
FwBamH1 CAF20	5′-CCGGATCCATGATCAAGTATACTATCGATG-3′
RvHindIII CAF20	5′-CCAAGCTTTTATGCTTCGTCGTCTTCGTC-3′
Fwcaf20T102A	5′-GAAGAAGAAACAGAAACCGCACCAACTTCTACTGTACCAG-3′
Rvcaf20T102A	5′-CTGGTACAGTAGAAGTTGGTGCGGTTTCTGTTTCTTCTTC-3′
Fwgga1::NATr	5′-GGACAAGTCACTACTTCAAGTATAACCCAGACAAGAGTCTTTTAAAACATGGAGGCCCAGAATACCC-3′
Rvgga1::NATr	5′-TCTCTGTAATATAATATGGCATCTACTTTTTTTTCAACTTCTCTACCGAATTTGACAGTATAGCGACCAGCATTCAC-3′
K2 gga1::NATr	5′-GGAGAAAACTTGTTAAGTCC-3′
Fwgga2::KanMX4	5′-CCCTGTTTTTTTTCTGTAATCACG-3′
Rvgga2::KanMX4	5′-CCTTTACACGATCTAGCATTGC-3′
K2 gga2::KanMX4	5′-CCCTTTTCAAGACAGTCTGA-3′