FIGURE 2.
RhoA acetylation interferes with F-actin formation. A, RhoGDIα acetylation induces actin polymerization in HeLaB cells. HeLa cells transiently transfected with RhoGDIα (bottom panels) or RhoGDIα-EGFP (top panels) were stained for filamentous actin (F-actin; red). B, quantitative analysis of F-actin. Shown is the F-actin fluorescence intensity of cells from A normalized to non-transfected cells. The results are depicted as mean ± S.D. (error bars) from three independent experiments. At least 10 cells were examined per condition per experiment. *, p < 0.01; **, p < 0.001 for the indicated comparison. #, p < 0.05 compared with RhoGDIαWT-expressing cells. For statistical analyses, a two-sided Student's t test was performed. Scale bars, 20 μm.