Analysis of collagen II processing.
a, sequence of the collagen II peptide found in the human lymph. b, Western blotting for membrane biotinylation in late endosomes (LE) and plasma membrane fractions (PM). c, Western blotting of ER, Golgi, and endosomal markers to check purity of plasma membrane preparation. Tot, total. d, Western blotting analysis for MMPs present in DC.