FIGURE 11.
At DIV4, inhibition of ribosomal biogenesis affects global protein synthesis. A and B, DIV1 hippocampal neurons were transfected as described for Fig. 10. On DIV4, cells were treated with 50 ng/ml BDNF for 45 min as indicated. Then OPP was added for an additional 30 min followed by fixation and visualization of nascent peptides as in Fig. 7, A and B. A, representative images of transfected neurons (arrows). B, in shS6-, shL4-, or shTIF1A-transfected cells, nascent peptide accumulation was significantly decreased. BDNF was unable to rescue such a deficit. Data represent means ± S.E. of at least 49 individual cells from three independent experiments; two-way ANOVA, effect of shRNA, F3,415 = 26.752, p < 0.001; post hoc tests: ***, p < 0.001; *, p < 0.05; NS, p > 0.05.