TABLE 1.

The QuikChange primers used in this study

The mutated bases are underlined in the sense primers.

Gene	Sense Primers	Antisense Primers
C157R	5′-GACGTAAAAAATGGAGAAGTTTTCGTGCTGACCATC-3′	5′-GATGGTCAGCACGAAAACGTTCTCCATTTTTTACGTC-3′
E71G	5′-GATGATATTGTAGACCGAGCAAAAATGGGAGATACCCTGAAGAGGAGGTTTTTCTATG-3′	5′-CATAGAAAAACCTCCTCTTCAGGGTATCTCCCATTTTTGCTCGGTCTACAATATCATC-3′
R420C	5′-CTCCTGTCATGCATGTGCCACCAAAGTCC-3′	5′-GGACTTTGGTGGCACATGCATGACAGGAG-3′
C468A	5′-GTATCTTGCCATTGCTGATGAGTGCTAC-3′	5′-GTAGCACTCATCAGCAATGGCAAGATAC-3′
R420C/C417A	5′-GCTGTTATGACCTCTCCGCTCATGCATGTGCCACC-3′	5′-GGTGGCACATGCATGAGCGGAGAGGTCATAACAGC-3′
C471A/C468A	5′-GTATCTTGCCATTGCTGATGAGGCATAC-3′	5′-GTATGCCTCATCAGCAATGGCAAGATAC-3′
C471A	5′-GCCATTTGTGATGAGGCATACATTACAGAAATG-3′	5′-CATTTCTGTAATGTATGCCTCATCACAAATGGC-3′
V513P	5′-CAGAAAACACTATATCCGGAAGAAGTTGTTCC-3′	5′-GGAACAACTTCTTCCGGATATAGTGTTTTCTG-3′
V441P	5′-CAGTCAATGTTCCTCAGTTTGAAC-3′	5′-GTTCAAACTGAGGAACATTGACTG-3′
G451V	5′-AAGGGAGCAATTGTTAAGGCATATAAG-3′	5′-CTTATATGCCTTAACAATTGCTCCCTT-3′
Del 432–436	5′-ACAGTCAATGTTGTTC-3′	5′-TTTCTCAGCTACAAG-3′
Del 447–456	5′-GATGCAAAACTGGTGATG-3′	5′-ACTGGGTTCAAACTGAAC-3′
Del Ins3	5′-CTATATGTGGAAGAAGAAGTTGTT-3′	5′-AACAACTTCTTCTTCCACATATAG-3′
Del Ins1	5′-AGCAAATTTGTCTACATGGCCAGAGG-3′	5′-AACTTAATGTTCAAGACTTT-3′