FIGURE 4.

REH2C is an mRNP. A–C, analyses as in Fig. 2A of REH2-IPs and 3010-IPs from GAP1-RNAi extract (A and B) and total mitochondrial (MITO) extract (C). KREPA1 (A1) is a core subunit of RECC. In A, total gRNA and mRNA RPS12 (FE, fully edited; PE, partially edited; UE, unedited) were assayed by ³²P capping and RT-PCR, respectively. Our cell line had two REL1 isoforms that were clearly resolved in some gels. D, densitometry of the proteins and gRNA in A. RGG2 was scored from B. Relative association was double-normalized to recovered REH2 in IPs and each component at day 0 (set at 1). E, densitometry of the proteins in C. Values were double-normalized to recovered REH2 and to day 0. F, -fold change in the levels of bound edited and unedited mRNAs in IPs in GAP1 RNAi cells. The ratio of mRNA in the IPs and total mtRNA input on days 3 and 4 of induction was compared with the same ratio in uninduced samples (day 0 set at 1). qRT-PCR calculations were normalized to a mock IP and input values. Standard deviation of the average value in Cq duplicates is the maximum deviation observed in the duplicates.