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Analysis of REH2 and 3010 purified complexes for unwinding activity and formation of an RNP with a short radioactive RNA duplex. A, assays of unwinding activity and RNP formation in REH2, 3010, and mock IPs with 2 mm ATP. B, radioactive autoadenylation of editing ligases in complexes that co-migrate with the RNPs in A. C and D, assays as in A but from H2F1-RNAi or GAP1-RNAi extracts on days 0, 3, and 4 of induction ± ATP.
