FIGURE 6.

Transcription start site and sequences of the gbpA regulatory region. A, transcription start site of gbpA was determined by primer extension of the RNA isolated from the wild type grown aerobically to an A₆₀₀ of 0.5. Lanes C, T, A, and G represent the nucleotide sequencing ladders of pKK1401. The asterisk indicates the transcription start site of gbpA. B, transcription start site of gbpA is indicated by a bent arrow, and the positions of the putative −10 and −35 regions are underlined. The sequences for binding of IscR (ISCRB1 and ISCRB2, white boxes), CRP (CRPB, shaded boxes), and SmcR (SMCRB, gray boxes) were determined later in this study (Fig. 10). The nucleotides showing enhanced cleavage are indicated by black boxes. The consensus sequences for binding of IscR (type 2), CRP, and SmcR are, respectively, indicated above the V. vulnificus DNA sequence. R, A or G; Y, C or T; W, A or T; x, any nucleotide.