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. 2016 Jan 11;291(11):5774–5787. doi: 10.1074/jbc.M115.685321

FIGURE 9.

FIGURE 9.

Sequences for binding of IscR, CRP, and SmcR to PgbpA. A 390-bp DNA fragment of the gbpA regulatory region was radioactively labeled and then used as a DNA probe. The radiolabeled DNA probe (25 nm) was incubated with increasing amounts of IscR (A), CRP (B), and SmcR (C) as indicated. The regions protected by IscR, CRP, and SmcR are indicated by white boxes, shaded boxes, and gray boxes, respectively. The nucleotides showing enhanced cleavage are indicated by black boxes. Lanes C, T, A, and G represent the nucleotide sequencing ladders of pKK1401.