TABLE 3.
SAXS parameters and statistics
| Data collection parameters | CASPR2–1261 |
|---|---|
| Instrument | SAXSess (Anton Paar) |
| Beam geometry | 10 mm slit |
| Wavelength (Å) | 1.5418 |
| q range (Å−1) | 0.01297–0.35140 |
| Exposure time (minutes) | 30 |
| Concentration range (mg ml−1) | 9.570–1.0 |
| Temperature (celsius) | 20 |
| Structural parameters | |
| Protein concentration (mg/ml) | 9.570 |
| I(0) (cm−1) [from P(r)] | 1.188 + 0.0060 |
| Rg (Å) [from P(r)] | 46.11 + 0.226 |
| I(0) (cm−1) [from Guinier] | 1.199 ± 0.007 |
| Rg (Å) (from Guinier) | 44.809 ± 1.633 |
| Dmax (Å) | 145 |
| Porod volume estimate (Å3) | 298,433 |
| Dry volume estimated from sequence (Å3) | 167,557 |
| Molecular mass determination | |
| Partial specific volume (cm3 g−1) | 0.7156 |
| Molecular mass, kDa (from I(0) compared with lysozyme) | 160 |
| Calculated monomeric mass from sequence (Da) | 138,551/153,229 (MS) |
| N-Linked glycosylation sites (potential) | 12 |
| Software employed | |
| Primary data reduction | Saxquant 1d |
| Data processing | Gnom |
| Ab initio analyses | Gasbor |
| Validation and averaging | Damaver |
| Rigid body modeling | Coral |
| Computation of model intensity | Crysol |
| Three-dimensional graphic representation | PyMol |