FIGURE 4.

Localization of nectin-1 and nectin-4 at the boundary between the luminal and basal cells distinct from desmosomes and gap junctions. A and C, localization of desmoplakin in the mammary gland of wild-type mice. Desmoplakin (red) was co-immunostained with nectin-1 or nectin-4 (green). B and D, localization of connexin 43 in the mammary gland of wild-type mice. Connexin 43 (red) was co-immunostained with nectin-1 or nectin-4 (green). E and F, localization of desmoplakin and connexin 43 in the mammary gland of nectin-1^−/− mice. Desmoplakin and connexin 43 was immunostained with the indicated Abs (red). Scale bars, 20 μm. Results are representative of five independent experiments.