FIGURE 6.

Functional impairments in pregnancy-induced alveolar development in nectin-1^−/− mammary gland. A, STAT5 tyrosine phosphorylation. The lysates (10 μg of protein each) of the mammary epithelium from non-pregnant (Non-P) and P18.5 wild-type and nectin-1^−/− mice were subjected to Western blotting using an anti-phospho-STAT5 (pSTAT5) Ab. Actin was used as a loading control. B, β-casein synthesis. The lysates (10 μg of protein each) of the mammary epithelium from non-pregnant and P18.5 wild-type and nectin-1^−/− mice were subjected to Western blotting using an anti-β-casein Ab. Results are representative of three independent experiments. IB, immunoblotting.