FIGURE 5.

The effect of crosslinker length and reactivity on SidM interactomes. Six crosslinking conditions were compared: no crosslinker, 1% formaldehyde, 1 mm DSP, 0.5 mm DTME, 1 mm SMCC, and 1 mm DSP/0.5 mm DTME. A, heat map of all identified proteins across all crosslinking conditions ranked based on enrichment factors calculated from the DSP condition. B, heat map showing the Top10 enriched proteins identified in SidM complexes found under DSP crosslinking conditions. Proteins also found ranked in the Top10 of their respective crosslinking condition are marked with a cross (X). C, Venn diagram comparing interactors identified by varying lysine-lysine crosslinking length. D, Venn diagram comparing SidM interactors identified by varying crosslinker reactivity.