Skip to main content
. 2016 Jan 11;291(11):5844–5859. doi: 10.1074/jbc.M115.675264

FIGURE 2.

FIGURE 2.

Sirt1 regulates PC1 and PC2 expression in N43-5 and AtT20 cells. A–C, N43-5 cells were transfected with 4 μg of Sirt1 cDNA to increase the expression of Sirt1, and cells were collected 48 h post-transfection. A, Sirt1 protein (n = 6/group). B, pro-PC2 (n = 6/group). C, pro-PC1 (n = 6/group). These results were repeated in an independent experiment with the same response. D–F, AtT20 cells were transfected with an shRNA targeted against Sirt1 or control shRNA and collected 72 h post-transfection. D, Sirt1 protein (n = 6 control shRNA and 11 Sirt1 shRNA). E, PC2 protein (n = 5 control shRNA and 7 Sirt1 shRNA). F, PC1 protein (n = 5 control shRNA and 7 Sirt1 shRNA). Data are mean ± S.E. *, p value < 0.05; **, p value < 0.01; ****, p value <0.00001.