FIGURE 5.

PVN Sirt1 activation enhances adrenal activity in lean rats. A–H, lean rats were intra-PVN-infused with SA3 (6 μg/rat) or vehicle beginning at 4 p.m. on day 1 and once more beginning at 10 a.m. the following day. Rats were sacrificed 3 h later. Trunk blood was collected, and serum was isolated and analyzed for parameters indicative of adrenal and thyroid activity and adenohypophysis analyzed for protein. A, GC (n = 9 vehicle and 11 SA3). B, ACTH (n = 4/group). C, T₃ (n = 7 vehicle and 9 SA3), and D, T₄ (n = 7 vehicle and 9 SA3). E–H, anterior pituitary was dissected and assayed by RIA for E. ACTH (n = 4 vehicle and 6 SA3) and by Western blot for F. POMC (n = 7 vehicle and 9 SA3); G, PC1 (n = 5 vehicle and 6 SA3);and H, CPE (n = 5 vehicle and 6 SA3). Data are mean ± S.E. *, p value < 0.05; **, p value < 0.01.