FIGURE 7.
PVN Sirt1 inhibition does not augment adrenal activity nor body weight in lean rats. Lean rats were intra-PV-infused with 5 μg of Ex-527 or vehicle control beginning at 4 p.m. on day 1 and beginning at 11 a.m. the following morning. Rats were sacrificed a few hours later, and PVN was collected and subjected to Western blotting analysis for protein measurement, and serum was isolated and analyzed for hormones indicative of adrenal and thyroid activity. A, PC2 (n = 5/group); B, PC1 (n = 5/group); and C, CPE (n = 6 vehicle and 7 Ex-527) protein. D and E, CRH was measured by RIA in D. PVN (n = 5 vehicle and 7 Ex-527) and E, ARC/ME (n = 6 vehicle and 8 Ex-527). F–J, serum analysis for F. GC (n = 6 vehicle and 5 Ex-527). G, T3 (n = 6 vehicle and 7 Ex-527). H, T4 (n = 6 vehicle and 7 Ex-527). I, glucose (n = 9 vehicle and 6 Ex-527). J, insulin (n = 7/group). K, net change in the amount of food consumed (n = 6 vehicle and 8 Ex-527). L, overnight oxygen consumption (n = 3 vehicle and 5 Ex-527), M, net change in overnight body weight (n = 6 vehicle and 8 Ex-527). Data are mean ± S.E.