FIGURE 2.

Secretion of LOR_107 with and without signal peptide. A and B, ulvan incubation with CM (A) or cell lysate (B) derived from overexpressed LOR_107 (red), LOR_107d (green, i.e. without signal peptide), and empty vector (black) was followed by UV (235 nm). Ulvan lyase activity is indicated by the increase in 235 nm absorbance. Error bars indicate ± S.E. C, Western blot of the CM fractions (LOR_107 (lane 1), LOR_107d (without signal peptide) (lane 2), and empty vector (lane 3)), and of cell lysate fractions (LOR_107 (lane 4), LOR_107d (lane 5), and empty vector (lane 6)).