TABLE 1.
Strains used in this study
| Strain | Relevant features | Reference |
|---|---|---|
| Ulvanolytic strains | ||
| N. ulvanivorans PLR | Ulvan-degrading strain, genome as a template | 6, 7 |
| Alteromonas sp. LOR | Ulvan-degrading strain, genome as a template | 12 |
| Alteromonas sp. LTR | Ulvan-degrading strain, genome as a template | 12 |
| Pseudoalteromonas sp. PLSV | Ulvan-degrading strain, genome as a template | 13 |
| E. coli strains | ||
| T7 express competent E. coli/pRIL (MK1466) | BL21 (DE3) enhanced derivate used for protein expression supplied with plasmid for rare codon usage, CmR | New England Biolabs/Stratagene |
| MK1466/pET22b | pET22b in MK1466, AmpR CmR | New England Biolabs/Novagen |
| MK1466/pET28a | pET28a in MK1466, KanR CmR | New England Biolabs/Novagen |
| MK1466/pMK2002 | pET22b_LOR_61 in MK1466, AmpR CmR | This study |
| MK1466/pMK2018 | pET22b_LOR_107 in MK1466, AmpR CmR | This study |
| MK1466/pMK2045 | pET22b_PLSV_3875 in MK1466, AmpR CmR | This study |
| MK1466/pMK2046 | pET22b_PLSV_3925 in MK1466, AmpR CmR | This study |
| MK1466/pMK1469 | pET22b_PLR_UL in MK1466, AmpR CmR | This study |
| MK1466/pMK2040 | pET22b_PLR_48 in MK1466, AmpR CmR | This study |
| MK1466/pMK2035 | pET28a_LOR_107 in MK1466, KanR CmR | This study |
| MK1466/pMK2036 | pET28a_LOR_107d in MK1466, KanR CmR | This study |