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. 2015 Dec 30;291(11):5879–5888. doi: 10.1074/jbc.M115.707802

TABLE 1.

Saccharomyces cerevisiae strains

Strain name Genotype Reference
yMD7 MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 bar1::Hyg This study
pep4::KanMX
MCM6::MCM6-3 × FLAG (Nat-NT2)
yMD8 MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 bar1::Hyg This study
pep4::KanMX
MCM6::MCM6-3 × FLAG (Nat-NT2)
his3::HIS3pRS303/CDT1,GAL4
trp1::TRP1pRS304/MCM4, MCM5 leu2::LEU2pRS305/MCM6TC, MCM7 ura3::URA3pRS306/MCM2, CBP-MCM3
yMD9 MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100, This study
cdc7-4, pep4:: Hyg,
MCM10::MCM10-3 × FLAG (Nat-NT2)
his3::HIS3pRS303/SLD3-13MYC,
trp1::TRP1pRS304/SLD2, leu2::LEU2pRS305/SLD7, CDC45,
ura3::URA3pRS306/DPB11
yMD16 MATa/α ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 MCM10+/mcm10Δ472-571 (Nat-NT2) This study
yMD17 MATa/α ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 MCM10+/mcm10Δ472-571 (Nat-NT2) RAD9/rad9Δ::KanMX This study