TABLE 2.
Plasmids generated in this study
| Name | Cloning vector | Insert | Generation of insert | 5′ site | 3′ site |
|---|---|---|---|---|---|
| pBP6 | pET28a | MCM10 | PCR (S. cerevisiae W303 genomic DNA template) | BamHI | BamHI |
| pMD84 | pET28a | MCM10 Δ473–571 | PCR (pBP6 DNA template) | BamHI | BamHI |
| pMD65 | pCS2-MYC-FA3 | MCM10 | PCR (pBP6 DNA template) | FseI | XbaI |
| pMD72 | pCS2-MYC-FA3 | MCM10 Δ1–371 | PCR (pBP6 DNA template) | FseI | XbaI |
| pMD73 | pCS2-MYC-FA3 | MCM10 Δ1–471 | PCR (pBP6 DNA template) | FseI | XbaI |
| pMD74 | pCS2-MYC-FA3 | MCM10 Δ473–571 | PCR (pBP6 DNA template) | FseI | XbaI |
| pMD75 | pCS2-MYC-FA3 | MCM10 Δ373–571 | PCR (pBP6 DNA template) | FseI | XbaI |
| pMD27 | pMAL-c2P | MCM6 789–1017 | PCR (full-length Mcm6 DNA template) | BamHI | SalI |
| pMD31 | pFA6a-kanMX6 | MCM6 790–1017 + TC site at Thr-870 | PCR (tcMcm6 DNA template) | BamHI | BamHI |