TABLE 2.

Values for unwinding rate constant, k_u (s⁻¹) obtained from data fitting using KinTek Global Kinetic Explorer

Substrate	DNA:DNA	RNA:DNA	Chimera I	Chimera II
16-bp forked duplex	71.2 ± 8.0a	74.0 ± 4.3a	NDb	ND
20-bp forked duplex	53.7 ± 2.4a	54.2 ± 4.5a	61.1 ± 7.6a	66.1 ± 1.4a
16-bp tailed duplex	68.8 ± 1.5a	72.5 ± 1.5a	ND	ND
20-bp tailed duplex	ND	56.4 ± 0.5a	ND	ND
24-bp forked duplex	65.7 ± 8.0a	66.8 ± 5.0a	ND	ND

^a Standard deviation of three independent experiments.

^b ND, not determined.