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Values for dissociation rate constant, kd (s−1) obtained from data fitting using KinTek Global Kinetic Explorer
| Substrate | DNA:DNA | RNA:DNA | Chimera I | Chimera II |
|---|---|---|---|---|
| 16-bp forked duplex | 2.8 ± 0.8a | 2.0 ± 0.3a | NDb | ND |
| 20-bp forked duplex | 4.5 ± 0.4a | 3.0 ± 0.2a | 3.8 ± 0.7a | 8.6 ± 0.7a |
| 16-bp tailed duplex | 11.2 ± 0.1a | 8.3 ± 1.3a | ND | ND |
| 20-bp tailed duplex | ND | 6.9 ± 0.4a | ND | ND |
| 24-bp forked duplex | 8.7 ± 1.3a | 6.3 ± 0.6a | ND | ND |
a Standard deviation of three independent experiments.
b ND, not determined.
