FIGURE 7.

Molecular status of endogenous AOP. A, recombinant AOP (50 ng) and subcellular fractions of KDP136 (2 μg of protein) were separated on SDS-polyacrylamide gels and subjected to immunoblotting. Lane M, full-length rainbow molecular weight marker; lane 1, recombinant WT AOP; lane 2, Asn¹⁰²; lane 3, KDP136 whole cells; lane 4, cytosol; lane 5, inner membrane; lane 6, periplasm/outer membrane. B, the periplasm/outer membrane fraction of KDP136 (0.3 ml) was loaded onto an HPLC column, as described in the legend to Fig. 6. Aliquots (5 μl) of separated fractions were subjected to SDS-PAGE and then visualized by immunoblotting with the anti-AOP antibody. p/o, periplasm/outer membrane fraction (5 μl).