TABLE 1.

Primers, plasmids, and DNA fragments used in this study

Restriction and mutated sites are shown in italic type and underlined, respectively.

Name	Sequence (5′–3′)	Product (plasmid or DNA fragment)
5PGN1349M16	ATGACTGTGCATGCACAAAAGATCA	pTrcHis2TOPO-PGN1349
3PGN1349-K759B	TTTCTTCAGGTGTTTGGCGAAGAAACC	pTrcHis2TOPO-PGN1349
5PGN1542-L6Bam	AAAAAAGGATCCCTACAGATCATCATACTGCT	pQE60-PGN1542
3PGN1542-E279Bam	AGATCTGGATCCTTCTACCTGAGATTCGATGT	pQE60-PGN1542
5PGN1878-I6Bgl	AAGCATAGATCTATTATTATCATCTAATGTAGT	pQE60-PGN1878
3PGN1878-L473Bgl	AGATCTAGATCTAAGACTATCCAGGAAGGAGC	pQE60-PGN1878
5PGN1349-N102	AATGTGGAGCAGATGGGCTTCAGCCT	pTrcHis2TOPO-PGN1349-N102
3pTrcHisTopo-L-1	AAGGGCCATGGTTTATTCCTCCTTAT	pTrcHis2TOPO-PGN1349-N102
5PGN1349-S615A	GCCCACGGTGGTTATGCCACGCTGAT	pTrcHis2TOPO-PGN1349-S615A
3PGN1349-A614	GGCACCGTATATGGCGATCCTGTCGG	pTrcHis2TOPO-PGN1349-N102-S615A
1349-5F1	ATAATCACCCAACAAAATACCAAGT	PGN1349 5′ region with cepA anchor
cep-comp-1349-5R	TGCTTCACACCATGGATATGATCATGTAGTCCTTC	PGN1349 5′ region with cepA anchor
1349-5F1-cepF	ATCATATCCATGGTGAAGCATCTTCGATGCTG	cepA with 5PGN1349 anchor
1349-3Fcomp-cep3R-comp	ATTGTTTTTGTTCCGATAGTGATAGTGAACGG	cepA with 5PGN1349 anchor
cep-3F-1349-3F1	TCACTATCGGAACAAAAACAATCCGCAGATCTTCGA	5PGN1349 3′ region with cepA anchor
1349-3Rcomp	GCTTTCAGCCTTATATCCTCCGGCTTA	5PGN1349 3′ region with cepA anchor
1349-5F2	ATGACTGTGCATGCACAAAAGATCA	5PGN1349-cepA
1349-3R2comp	TTTCTTCAGGTGTTTGGCGAAGAAACC	5PGN1349-cepA