TABLE 3.
Enzymatic parameters of P. gingivalis exopeptidases
| Peptidase | MCA peptide | kcat | Km | kcat/Km | Source/Reference |
|---|---|---|---|---|---|
| s−1 | μm | μm−1 s−1 | |||
| AOP | Z-Lys-Met- | 1155 ± 77 | 17.8 ± 2.0 | 65.0 ± 3.7 | This study |
| Z-VKM- | 592 ± 40 | 4.9 ± 1.0 | 123.3 ± 17.3 | This study | |
| Z-AVKM- | 399 ± 13 | 6.9 ± 0.6 | 57.5 ± 3.7 | This study | |
| DPP4 | Gly-Pro- | 6917 ± 1253 | 100.9 ± 20.1 | 66.8 ± 2.0 | This study |
| DPP5 | Lys-Ala- | 1948 ± 165 | 185 ± 21 | 10.5 | Ref. 24 |
| DPP7 | Met-Leu- | 394 ± 79 | 39.6 ± 16.0 | 10.6 ± 2.5 | Ref. 25 |
| DPP11 | Leu-Asp- | 10,707 ± 140 | 19.5 ± 0.4 | 547.4 ± 6.3 | This study |
| Leu-Glu- | 13,587 ± 577 | 80.9 ± 5.7 | 167.7 ± 5.3 | This study |