TABLE 1.
Malachite green dephosphorylation assay examining AtRLPH2 activity towards phosphorylated peptides
Assays were performed in triplicate using 1 mm phospho-peptide and 1 μg of bacterially expressed and purified AtRLPH2-V5-H6. Phosphorylated amino acids are shown in bold. All peptides are derived from human sequences except rSAPK3, which is rat SAPK3. Values are followed by ± S.E. (n = 3).
| Peptide origin | Peptide sequence | nmol of Pi min−1 mg−1 |
|---|---|---|
| RRP1B | SSKKVpTFGLN | 0.997 ± 0.009 |
| BRCA1 | QSPKVpTFECEQK | 1.92 ± 0.03 |
| Ki67 | KRRRVpSFGGH | 1.479 ± 0.004 |
| B56 | KLQGIDpSQGVNQ | 1.82 ± 0.03 |
| HIPK4 | VKEPpYIQSR | 17.3 ± 0.4 |
| HIPK | VCSTpYLQSR | 18.3 ± 1.4 |
| hSAPK4s | EMTGpYVVTR | 18.8 ± 0.5 |
| PINK1 | RAHLESRSpYQEAQLPAR | 32.4 ± 0.6 |
| ICK | KPPYTDpYVSTR | 37.6 ± 0.9 |
| DAPP1 | PRKVEEPSIpYESVRVH | 49.8 ± 1.2 |
| hSAPK4 | RHADAEMTGpYVVTR | 99.7 ± 1.6 |
| rSAPK3 | RQADSEMTGpYVVTR | 103.0 ± 1.6 |