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. 2016 Mar 8;7:10909. doi: 10.1038/ncomms10909

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(a) Co-immunoprecipitation of Syn-4 with different Xcad-11 constructs. (First row) Precipitation of Syn-4 with FLAG antibody from transfected HEK293 cells. Western blot for Xcad-11 (myc) showed successful co-precipitation of Xcad-11, Xcad-11 Δe and Xcad-11 ΔeΔc. In the case of Xcad-11 ΔeΔTΜ and pCS2+, no co-precipitation with Syn-4 was detected. (Second row) Input for the different Xcad-11 constructs was detected by western blotting. (Third row) Precipitation of different Xcad-11 constructs with myc antibody from transfected HEK293 cells. Western blot for Syn-4 (FLAG) showed successful co-precipitation of Xcad-11, Xcad-11 Δe and Xcad-11 ΔeΔc. In the case of Xcad-11 ΔeΔTΜ and pCS2+, no co-precipitation with Syn-4 was detected. (Fourth row) Input for Syn-4 was detected by western blotting. Transfection of Xcad-11 alone served as negative control (first panel). (b) Co-immunoprecipitation of Syn-4 with different Xcad-11 constructs analysed for β-catenin (βcat) binding. (First row) Precipitation of different Xcad-11 constructs with myc antibody from transfected HEK293 cells. Western blot for βcat showed successful co-precipitation of Xcad-11, Xcad-11 Δe and Xcad-11 ΔeΔTΜ . In the case of Xcad-11 ΔeΔc and pCS2+, no co-precipitation with βcat was detected. (Second row) Precipitation of Syn-4 with FLAG antibody showed successful co-precipitation of βcat when Xcad-11 or Xcad-11 Δe were co-transfected. (Third row) Input for βcat was detected by western blotting. Transfection of Xcad-11 alone served as negative control (first panel). (c) NCC explants co-injected with mbGFP and Syn-4 MO and immunostained for phosphopaxillin. Depletion of Syn-4 leads to loss of phosphopaxillin staining, which is rescued by co-injection of Syn-4. Scale bars, 20 μm.