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. 2016 Mar 11;6:22921. doi: 10.1038/srep22921

Figure 4. CQ induces DR5 up-regulation through mRNA stabilization in Caki cells.

Figure 4

(a) Caki cells were treated with indicated concentrations of CQ for 24 h. The mRNA level of DR5 was determined by RT-PCR (upper panel) and qPCR (lower panel). The level of actin was used as a loading control. (b) Caki cells were treated with 30 μM CQ for the indicated time periods. The mRNA level of DR5 was determined by RT-PCR (upper panel) and qPCR (lower panel). The level of actin was used as a loading control. (c) Caki cells were transiently transfected with a plasmid harboring the luciferase gene under the control of the DR5/Sac I and DR5/-605 promoter. After transfection, the Caki cells were treated with 30 μM CQ for 24 h. After treatment, the cells were lysed, and the luciferase activity was analyzed. (d,e) Caki cells were treated 30 μM CQ for 12 h, then changed with fresh medium and pre-treated with 5 μg/ml actinomycin D (Act D) for 30 min, and treated with or without 30 μM CQ for the indicated time periods. The mRNA level of DR5 was determined by RT-PCR (d) and qPCR (e). The values in panel (a–c) represent the mean ± SD from three independent samples. *p < 0.05 compared to the control.