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. 2016 Mar 11;6:22976. doi: 10.1038/srep22976

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(A) qRT-PCR analysis of the basal c-Jun expression in HCC cell lines; (B) Relative mRNA expression levels of c-Jun on PLC/PRF/5 and SMCC7221 cells were detected by real-time polymerase chain reaction (PCR) at transcriptional level. Data shown are the means (SD) from at least three independent experiments; (C) The expression of c-Jun and p-c-Jun increase after treated with sorafenib in PLC/PRF/5 cells in a time-dependent manner by western blot analysis.