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. 2016 Mar 9;7:10940. doi: 10.1038/ncomms10940

Figure 1. A Trp-BODIPY fluorogenic amino acid.

Figure 1

(a) Synthetic scheme and spectral properties of the Trp-BODIPY fluorogenic amino acid 3 (NR: no reaction). (b) The amino acid 3 displays strong fluorogenic behaviour in phospholipid membranes. Spectra of compound 3 (10 μM) were recorded after incubation with PC:cholesterol (7:1) liposome suspensions in PBS ranging from 3.75 to 0.004 mg ml−1 of PC in two-fold serial dilutions, λexc.: 450 nm. PBS alone was used as a negative control for a non-hydrophobic environment. On the right-hand side, pictures of the fluorescence emission of 3 under excitation with a 365 nm UV-lamp in PC:cholesterol liposome suspensions with increasing PC content (from top to bottom: 3.75, 1.88, 0.94, 0.47, 0.23 and 0 (only PBS) mg ml−1 of PC).