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. 2016 Mar 9;7:10940. doi: 10.1038/ncomms10940

Figure 3. The cyclic peptide 8 is a highly stable fluorogenic agent for high-resolution imaging of A. fumigatus.

Figure 3

(a) Comparative chemical stability of mono-labelled BODIPY linear (5) and cyclic (8) PAF26 analogues in human bronchoalveolar lavage samples from patients with acute respiratory distress syndrome. (b) Chemical structure of the cyclic BODIPY-labelled peptide 8. (c) Kinetic analysis (from time-lapse imaging in d of the fluorescence signal of compound 8 (2 μM) in the cell membrane of A. fumigatus (arrow points at the addition time for compound 8). (d) Time-lapse high-resolution imaging of A. fumigatus upon incubation with a cell membrane counterstain (red) and compound 8 (2 μM, green) for 0 min (i), 1 min (ii), 3 min (iii) and 10 min (iv) (see Supplementary Movie 3). Scale bar, 2.5 μm.